Pluskal Biotech Consulting
R&D, Product Development, Purification, IP Technology assessment, Education, Market Research
Technical Skills
Consulting Services has a wide range of technical expertise available in the Biotechnology application area.   Dr. Pluskal has maintained a close association with Boston Biomedical Research Institute (www.bbri.org) where he is able to carry out application projects.   Consulting Services can offer a range of services from "hands on" bench level support and troubleshooting to executive level technology assessment and business development support.   Listed below are a short summary of specific areas where skills are available from Consulting Services.

PROTEOMICS
                                                  

           - Sample complexity reduction
           - Abundant protein depletion
           - 2DE electrophoresis analysis
           - Western blotting
           - Preparative IEF
           - Protein array chemistry and content selection
           - In-gel digest and MALDI-MS
           - Commercial product development 
           - Automation

MOLECULAR BIOLOGY

         - DNA/RNA isolation from clinical samples
         - PCR and Real time qPCR
         - Detection of contaminating NA in process samples
         - Detection of rare cells in clinical samples
         - Primer design and database searching
         - Oligonucleotide synthesis
         - Hybridization kinetics and array hardware design

CHROMATOGRAPHY

         - Design of IEX and affinity media and manufacture
         - Production of MAb at cGMP scale
         - Ligand coupling to activated supports 
         - Production of Protein A and thiophilic affinity supports
         - Sample complexity reduction for Biomarker Discovery
         - Abundant protein removal
         - Interface with MALDI-MS and LC-MS/MS

CHEMISTRY

         - Surface modification of membrane surfaces
         - Formation of particles
         - Activation chemistries
         - Peptide coupling under cGMP
         -
Coating of magnetic particles for surface activation
         - Understanding of aqueous hydrogel polymerization
                  - IPG strip manufacture
                  - Pre-cast SDS-PAGE manufacture
         - Affinity ligand synthesis at production scale
                  - Protein A supports
                  -
Thiophilic ligands
         - Small scale organic synthesis of unique monomers
         - Custom solid phases for oligonucleotide synthesis
         - Polymer surface modification for MALDI-MS applications

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