Pluskal Consulting Services has wide experience of protein purification for research, crystallography, antibody induction, diagnostic reagents and immunoaffinity applications at lab scale, through to pilot at GLP and cGMP levels of regulation. In addition to isolation from native sources, experience has been gained in protein and yeast based expression systems. In the later case considerable experience has been gained in protein re-folding and characterization. A summary of specific skills are listed below;
- cGMP production of protein biologic's
- antibody purification
- bacterial and yeast expression systems
- Bioprocess chromatography
- Creation of methodology and documentation to support production of antibodies by Protein A based purification
- Production at up to 100 g scale from bioreactors
- Creastion of antibody-hapten conjugates
- Creation of immunoaffinity reagents
- Protein product characterization
- Electrophoresis
- Mass spectrometry
- Carbohydrate analysis
- Light scattering
- Differential scanning calorimetry
- Size exclusion/GPC analysis of polymers
