Malcolm G. Pluskal, Eva Golenko and Mary F. Lopez Applications of Ion-Exchange (IEX) chromatography to reduce sample complexity prior to two-dimensional gel electrophoresis (2DE) in Separation methods in Proteomics (CRC Press) edited by Gary B. Smejkal and Alexander Lazarev, pg 19 - 28 (2006)
Malcolm G. Pluskal and Jutta Bachman. Advancing Applications of Microarrrays Drig Doscovery Today 9, pg 61-63 (2004)
Lopez MF, Pluskal MG. Protein micro- and macroarrays: digitizing the proteome. J Chromatogr B Analyt Technol Biomed Life Sci. (2003) Apr 5;787(1):19-27.
Pluskal MG, Bogdanova A, Lopez M, Gutierrez S, Pitt AM : Multiwell in-gel protein digestion and microscale sample preparation for protein identification by mass spectrometry. Proteomics (2002) Feb;2(2):145-50
Malcolm G. Pluskal. Microscale sample preparation: The demand for microscale analysis has stimulated the development of new solid phase formats in sample preparation. Nature Biochemistry 18 Vol. 1, 104-105 (2000)
Michael A. Mansfield, Masaharu Mabuchi, Constance G. MacDonald and Malcolm G. Pluskal. Immobilon? -Ny+ Nucleic acid blotting membrane: An advanced nylon membrane optimized for superior fixation and reprobing. BioTechniques 27: 1253-1257 (1999)
Enal S. Razvi, Liming Ge, Leodevico L. Ilag, Kambiz Amdjadi and Malcolm G. Pluskal. Proteomics: An overview and analysis of current and emerging technologies, the competitive landscape and market environment. D&MD reports #1992, October 1999.
Malcolm G. Pluskal (1999) Proteomics- Technology Integration for Target Discovery Drug & Market Development Reports 10, 243-247.
Malcolm G. Pluskal (1999) Developing Antisense-Based Therapeutics: Where are we today? Drug &Market Development Reports 10, 102-107.
Malcolm G. Pluskal (1997) Recent Developments of Biochip Technologies in DNA-Based Research and Diagnostic Applications. Drug & Market Development 9, 43-47.
Malcolm G Pluskal (1997) DNA Purification Systems: A Report on the Current Status and Future Prospects. Life Science Advertising News 2, 15-17.
J. Charkoudian, M.G. Pluskal and R.Horton. (1995) Membrane Surface for Carbohydrate Analysis. In Analytical Letters 28, pg. 6-12. P. Jackson, M.G. Pluskal and W.M. Skea. (1994) The Use of Polyacrylamide Gel Electrophoresis for the Analysis of Acidic Glycans Labeled with the Fluor 2-Aminocridone. In Electrophoresis 15, pg. 896-902.
W.R. Patton, M.G. Pluskal, W.M. Skea, J.L. Buecker, M.F. Lopez, R. Zimmermann, L.M. Belanger and P.D. Hatch (1990) Development of a Dedicated Two-Dimensional Gel Electrophoresis System that Provides Optimal Pattern Reproducibility and Polypeptide Resolution. Biotechniques 8:518-527.
Edgar Lauritzen and Malcolm G. Pluskal. (1988) Improved HIV Antiglycoprotein Antibody Detection by Immunoblotting on a Hydrophobic Membrane. Journal Acquired Immune Deficiency Syndromes. 1:333-339.
Malcolm G. Pluskal, Michelle B. Przekop, Mark R. Kavonian, Charles Vecoli and David A. Hicks (1986) Immobilon PVDF Transfer Membrane: A new Membrane Substrate for Western Blotting of Proteins. Biotechniques 4:272-283.
M.J. Gemski, B.P. Doctor, M.K. Gentry, M.G. Pluskal and M.P. Strickler. (1985) Single Step Purification of Monoclonal Antibody from Murine Ascites and Tissue Culture Fluids by Anion Exchange High Performance Liquid Chromatography. Biotechniques 3:378-384.
PATENTS
1. US patent application publication "Coated hydrophilic membranes for electrophoresis applications", US20060076237A1 published April 13th, 2006.
1. US patent application publication "Array based biomolecule analysis", US20040115834A1 published June 17th, 2004 and US20050019942A1 published January 27th, 2005.
2. Method and Apparatus for Covalent Immobilization of Charge-Conjugated Carbohydrate Molecules. John Charkoudian, Ronald Horton, Dave Wang and Malcolm G. Pluskal. US patent #5,011,861 (1996)
3. Method and device for rapid purification of biomolecules using ion exchange membranes. Yolanda Nieuwkerk, Robert J. Barry, Malcolm G. Pluskal and Richard Hamilton. US patent #5,438,128 (1995)
4. Charge-Modified Hydrophobic Membrane Materials and Method for making the same. Malcolm G. Pluskal, Michael Steuck and David Wang. US patent #5,004,543(1991)
5. Membranes for Solid Phase Protein Sequencing. James C. Coull, Darryl J. C. Pappin, Hubert Koester, Malcolm G. Pluskal, Michael Steuck and Alex G. Bonner. US patent #5,011,861 (1991)
SEMINARS/PRESENTATIONS
Chemical Affinity Ligands for Proteomics: Bioprocess Applications. Malcolm G. Pluskal, Ph.D. At BioPhex Sept 28-30, 2003 in San Jose, CA.
Application of novel affinity ligands for removal of abundant proteins from serum or plasma samples prior to high resolution Proteomic analysis. Mary Lopez, Sara Gutierrez, Malcolm G. Pluskal, Alla Bogdanova, Rebecca Doyle and Aldo M. Pitt. ASMS meeting (2002) Orlando, FL
High throughput in-gel peptide digestion and microscale sample preparation for MALDI ToF MS analysis. Malcolm G. Pluskal, Alla Bogdanova, Mary Lopez, Sara Gutierrez and Aldo M. Pitt Poster presented at ABRF meeting San Antonio, TX (2001)
Active Esters of Phenylpyrazolinones for the Labeling of Biomolecules. Nanda D. Sinha, Malcolm G. Pluskal and Jonathan N. Kremsky. Presented at the 24th A.C.S. Northeast Regional Meeting, Burlington, VT June 19-22, 1994.
Ion-Exchange Separation of Complex Biomolecules in Ultrafree®-MC Devices Containing Charge Derivatized Membranes. Tutorial presented at the 85th Annual Meeting, American Society for Biochemistry and Molecular Biology, Washington, DC May 23rd, 1994.
Peptide Nucleic Acid Probes and their Application in DNA and RNA Blot Hybridization Analysis. M. Pluskal, M. Mansfield, H. O'Keefe, C. Kissinger and B. Alzner-DeWeerd. Poster presentation at the 85th Annual Meeting, American Society for Biochemistry and Molecular Biology, Washington, DC May 23rd, 1994.
